urgent help please

Louis Hom lhom at OCF.Berkeley.EDU
Tue May 30 07:43:39 EST 2006


And actually, having 10 mM MgCl2 on its own (in the absence of EDTA of 
course) will make the DNA quite easy to pellet so that you can just spin 
it out instead of shearing it or digesting it.  However, I strongly 
recommend adding it at the same time as the lysozyme, NOT after the cells 
are already lysed.  Granted, it makes it harder to tell when the cells 
have lysed, but it works really well for me.
-- 
______________________________________________________________________________
Lou Hom >K'93			     
lhom at ocf.berkeley.edu		
http://www.ocf.berkeley.edu/~lhom/ 	    


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