5' site mapping, phosphatase

aru thinkofaru at rediffmail.com
Tue May 30 00:12:06 EST 2006


i am trying to identify 5' site of a mitochondrial mRNA. since these
transcripts do not carry cap at 5' ends i want to know whether CIP (
Calf intestine phosphatase) can remove all three phosphate groups (
i.e. gamma, beta and alpha phosphates) from the first ribo-nucleotide
of mRNA.
          thanx.



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