urgent help please

Duncan Clark blackhole at abuse.plus.com
Tue May 30 11:32:32 EST 2006


Historians believe that in newspost 
<mailman.18.1149002177.18505.methods at net.bio.net> on Tue, 30 May 2006, 
chiranjit chowdhury <cjtcdy at gmail.com> penned the following literary 
masterpiece:
>You have sent a protocol in which you suggested to do centrifugation at
>45000rpm using 45Ti rotor (Beckman).But I don't have access to this
>ultracentrifuge.We are having Sorvall ultracentrifuge.Please tell me in
>which rcf(xg)I should I have to centrifuge and which rotor (vertical,fixed
>angle or swing rotor )should I have to use.

Vertical and swing out primarily for gradient i.e. CsCl, Sucrose etc.

Fixed angle for removing debris.

Find the details for the 45Ti rotor and then recalculate the equivalent 
rpm for the same rcf in whatvere rotor you are using.

Duncan
-- 
I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.


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