strange size of protein using pet22b vector

faiza gul faiza_phd at yahoo.com
Wed May 31 04:24:59 EST 2006


hi.
  I am working on mammalian hormone.this protein gives very good expression band of total cell fraction and also gives band in extracellular and periplasmic fraction. but problem is this that the protein size is 3-4kd bigger than expected weight.please guide me in order to get protein band at its original size.
  waiting for your reply.
  thanks
   
  faiza
  punjab university,
  lahore,
   

		
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