DNA quality

Chrissy and Nina chrissyandnina at yahoo.co.uk
Wed May 31 11:39:56 EST 2006

Are you trying to use your DNA in a commercial PCR-SSP / PCR-SSOP or is 
it a PCR you have devised yourself. The KIR receptors are very hard to 
work with as the genes that encode them are very highly conserved and 
often what you think may be poor DNA quality can be bad primer design. 
If you are using long template OCR then the potential for the DNA to be 
the problem is increased but the LT-PCR kits tend to work only 
sporadically and I would investigate other kits or buffers if you can.

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