same protein produced in different cell lines

Pow Joshi via methods%40net.bio.net (by pow.joshi At gmail.com)
Sat Nov 4 17:05:38 EST 2006


On 3 Nov 2006 15:59:26 -0800, ointment <jeimysb At gmail.com> wrote:
> Hi all -
>
> I'm testing two versions of the same protein, given to me by two
> collaborators. One was produced in BHK cells (stable transfection), and
> the other in COS-7 cells (transient transfection). I was trying to test
> how these constructs bind to my target protein, and strangely enough,
> they are behaving differently in the binding assays.

could you please specify how different is different?

pow

 Does anyone have
> insight on why this might be happening, i.e., differential
> glycosylation? Thanks!
>
> Samira
>
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