same protein produced in different cell lines

[WS]

Dear Samira,

from a pessimistic point of view: Are you sure the 2 samples are really
identical - DNA-/protein sequence (any tags?), purity?, concentration,
buffer & preservatives present?

As you are concerned about different glycosylation: You might check it
by running samples in parallel on a SDS-Gel, and with / without
enzymatic or chemical deglycosylation.

The proof then might be asking your collaborators to exchange plasmids
and express them in their respective cells. Then check again.

Wo

ointment wrote:
> Hi all -
>
> I'm testing two versions of the same protein, given to me by two
> collaborators. One was produced in BHK cells (stable transfection), and
> the other in COS-7 cells (transient transfection). I was trying to test
> how these constructs bind to my target protein, and strangely enough,
> they are behaving differently in the binding assays. Does anyone have
> insight on why this might be happening, i.e., differential
> glycosylation? Thanks! 
> 
> Samira