Lyophilizing sterile samples in a Speed-Vac

[WS]

You could try this workaround:

deprive some eppi lids from their tubes and cut out circular holes.
Then use them to clamp a piece of suitable membrane on the vial where
your solution is in. That's it. I used this setup some time ago to
convert eppis into dialysis tubes.

Wo

netgk netgk wrote:
> I used to use Eppendorf's LidBac tube for lyophilizing sterile samples of
> growth factors and other expensive, labile molecules in a Speed Vac.  It has
> a sterilizing filter that removes microbes from the air that rushes in once
> the vacuum is released.  However, they will soon stop marketing this
> product.  Does anyone know of another way to keep sterile solutions from
> becoming contaminated during their time in the Speed Vac?
>
> I wish someone else would pick up the ball and improve on Eppendorf's
> design, which was not really intended for this purpose.  Ideally, the filter
> should be in the cap of the tube, not in a canister inserted into the tube.
> A cap filter would make it much easier to use, because one would not have to
> handle the canister (inserting and removing), which risks contaminating it.
>
> Cheers,
>
> Geoffrey Kidd
>
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