PCR band smear

[Duncan Clark]

Historians believe that in newspost 
<mailman.8.1163443809.19683.methods At net.bio.net> on Sun, 12 Nov 2006, 
muhammad yasir <yasirphr At yahoo.com> penned the following literary 
masterpiece:
>i am running PCR unsing PCR product as templet and facing the problem of smear at extactly the same position aroung of desired band size. Any
>sugestion for this problem

How much are you diluting the original PCR product and how many cycles 
are you then doing ?

Duncan
-- 
I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.