PCR band smear

[Domo]

Ta ==> 60C for 30 sec

increase annealing temperature

"muhammad yasir" <yasirphr At yahoo.com> wrote in message 
news:mailman.8.1163443809.19683.methods At net.bio.net...
>i am running PCR unsing PCR product as templet and facing the problem of 
>smear at extactly the same position aroung of desired band size. Any 
>sugestion for this problem
>  with thanks
>  Yasir
>
>
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