Question about protein dialysis-bag leakage

DY via methods%40net.bio.net (by bearofthecity At gmail.com)
Thu Nov 16 17:14:25 EST 2006


I don't know whetehr anyone have encountered the same strange problem.
I have one protein that is purifified from Ni column. The major peak is
eluted with about 250 mM immidazole. I tried to dialyze this protein to
some other buffer. The remarkable thing is once I add protein solution
into the dialysis bag, the bag starts to leak!
The bag is not leaky initially, as checked by only adding buffer into
it. Therefore, the protein definitely plays a role in this leaky issue.

Have anyone seen anything like this? I am really puzzled because this
seems so counter intuitive.



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