Methods Digest, Vol 18, Issue 15

[Virash Gupta]

No need to decontaminate as PCR reaction mix in closed tubes can not
contaminate the PCR block except for when PCR tubes are wrongly
handeled during reaction setup. Some PCR machines may loose efficiency
due deposition of dust or dirt with time. In such a case, for to be
sure of cleanlness, wipe inside of PCR block holes with a mild
detergent sol, let it stand for 5-10 min and then wipe with clean
water and 70 % Etanol followed bydrying with kimwipe or other nonfibre
relasing paper. You need to do it manually using cotton swab made on a
metal wire.
V K Gupta

On 11/16/06, methods-request At oat.bio.indiana.edu
<methods-request At oat.bio.indiana.edu> wrote:
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>   1. Re: UV and the PCR machine? (Jose de las Heras)
>   2. Re: UV and the PCR machine? (DK)
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> ----------------------------------------------------------------------
>
> Message: 1
> Date: Thu, 16 Nov 2006 00:33:02 -0000
> From: "Jose de las Heras" <josenet At tiscali.co.uk>
> Subject: Re: UV and the PCR machine?
> To: methods At net.bio.net
> Message-ID: <4s1pv0Ft46iuU1 At mid.individual.net>
>
>
> "Nenad Malenica" <malenica At biol.pmf.hr> wrote in message
> news:mailman.37.1163602169.19683.methods At net.bio.net...
> > Hello there,
> > I plan to "decontaminate" my  PCR machine (the block) by  exposing it to
> > UV overnight (in the laminar I have a 30W UV lamp). But I am not sure if I
> > can harm the machine in any way by this treatment.  I suppose HCl or NaOCl
> > (bleach) is out of the question (to clean the block itself). Any
> > suggestions?
> > Nenad
>
> why do you want to do that?
>
> the reactions are mixed and the tubes closed by the time they come near the
> machine. I'd have assumed the chances of contamination sourced to the
> machine would be practically non-existent.
>
> Jose
>
>
>
>
> ------------------------------
>
> Message: 2
> Date: Thu, 16 Nov 2006 02:40:25 GMT
> From: dk At no.email.thankstospam.net (DK)
> Subject: Re: UV and the PCR machine?
> To: methods At net.bio.net
> Message-ID: <scQ6h.47$6e4.21 At newsfe02.lga>
>
> In article <4s1pv0Ft46iuU1 At mid.individual.net>, "Jose de las Heras" <josenet At tiscali.co.uk> wrote:
> >
> >"Nenad Malenica" <malenica At biol.pmf.hr> wrote in message
> >news:mailman.37.1163602169.19683.methods At net.bio.net...
> >> Hello there,
> >> I plan to "decontaminate" my  PCR machine (the block) by  exposing it to
> >> UV overnight (in the laminar I have a 30W UV lamp). But I am not sure if I
> >> can harm the machine in any way by this treatment.  I suppose HCl or NaOCl
> >> (bleach) is out of the question (to clean the block itself). Any
> >> suggestions?
> >> Nenad
> >
> >why do you want to do that?
> >
> >the reactions are mixed and the tubes closed by the time they come near the
> >machine. I'd have assumed the chances of contamination sourced to the
> >machine would be practically non-existent.
>
> Seconded.
>
>
>
> ------------------------------
>
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> End of Methods Digest, Vol 18, Issue 15
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>