What would you reccommend as a WB marker (ladder) standard
Lechu
via methods%40net.bio.net
(by lech_kaczmarczyk At yahoo.com)
Fri Nov 17 17:29:05 EST 2006
WS napisal(a):
> My favourites are the prestained ladders, so you can see the proteins
> separating during the run and stop the electrophoresis when it looks
> promising. Then, after blotting you may easily mark the bands in case
> they disappear. I personally like the PageRuler mixes from Fermentas.
> Other companies have similar stuff that's also good.
well, I also used page ruler as a prestained marker, but now I meant
sth that would be detected on the blot, for instance by my secondary Ab
(IgG-HRP). Thanks anyway, Lech
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