Expression under T7 Control with Rifampicin?

SJC via (by scott.coutts At
Wed Nov 22 04:08:36 EST 2006

asavel wrote:
> Hi there,
> I'm looking for a protocol to puls-express a protein selectively. The
> protein is under control of a T7 promotor and I recall that I once had
> a protocol that used rifampicin to selectively shut off the coli
> RNA-Pol so that only T7-controlled protein expression takes place. This
> was from a protocol for S-35 labeling of proteins.
> Any ideas? 

Try this:


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