Expression under T7 Control with Rifampicin?

SJC via methods%40net.bio.net (by scott.coutts At med.monash.edu.au)
Wed Nov 22 04:08:36 EST 2006


asavel wrote:
> Hi there,
> 
> I'm looking for a protocol to puls-express a protein selectively. The
> protein is under control of a T7 promotor and I recall that I once had
> a protocol that used rifampicin to selectively shut off the coli
> RNA-Pol so that only T7-controlled protein expression takes place. This
> was from a protocol for S-35 labeling of proteins.
> 
> Any ideas? 
> 

Try this:

http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1166907

Scott.


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