Western Blot

Maryam via methods%40net.bio.net (by mzargh At yahoo.ca)
Thu Nov 23 19:18:41 EST 2006

I have an affinity purified antibody that is reacting with the expressed recombinant protein from bacteria in wetern blot but not with the normal fibroblast. This antibody was working very well with the fibroblast previously.
   Any idea how this can happen?
  Thanks in advance.

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