4 western blots

SJC via methods%40net.bio.net (by scott.coutts At med.monash.edu.au)
Fri Nov 24 06:51:18 EST 2006


DanSolo wrote:
> Lechu wrote:
>> Is there any brand you would (not) reccommend? I was thinking of Sigma
>> (mol.biol.grades), but there are so many much (more than 10 times
>> sometimes) cheper manufacturers (like Roth in Germany for example). I
>> just wonder whether this stuff is eqally good, especially for westerns
>> with really expensive Abs.
>>
>> Best wishes, lech
> 
> It's pretty basic stuff, nitrocellulose, so I for the cheapest I can
> find at .2uM pore size. At the moment I'm using a Schleiser and Scheull
> roll. I've never seen an advantage with PVDF, but some people like it.
> 

I like PVDF because it's a bit stronger, it's non-flammable, and you can 
dry it rather than block it (although I dont do that often, but only 
because of old habits!)

Scott.


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