siRNA transfection of primary neurons

StewJW via methods%40net.bio.net (by stewjw At gmail.com)
Mon Nov 27 05:01:59 EST 2006


Here are some references from Invitrogen cell line database:
Source: Nucleic Acids Res 2001 May15;29(10):2052-8
Comments: SK-N-SH cells plated on glass coverslips were transfected
with different...
Scientific Application(s): Transient Transfection
Product Name and Cat No: Lipofectamine™ 2000 (11668019)
 3. Source: Proc Natl Acad Sci U S A 2002 99(23)14716-14721
Comments: SK-N-SH cells were stably transfected with Lipofectamine
2000.
Scientific Application(s): Stable Cell Transfection
Product Name and Cat No: Lipofectamine™ 2000 (11668019)

They also have a protocol but thats for Invitrogen proprietary Stealth
RNAi which will be easier to transfect.

Invitrogen have a new transfection product Lipofectamine™ RNAiMAX
Transfection Reagent designed for siRNA but I have no info for neuronal
cells. Maybe worth giving a try.



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