SDS-PAGE with small proteins

Yoram Gerchman via methods%40net.bio.net (by gerchman At research.haifa.ac.il)
Mon Nov 27 12:47:06 EST 2006


> Hi everybody,
> I wonder if anyone knows how to separate and visualise small proteins
> (4-10 kDa) on a gel. I tried gradients, high percentage gels and
> Tris-tricine gels w/o any luck. When I stain with Coomassie or Ponceau
> (after transfer onto 0.2 u nitrocellulose) I can see nice bands up from
> 10 kDa. Below that point nothing shows up! Also, I tried blotting with
> specific abs w/o any success. I would appreciate any suggestions.
> Cheers, Marcin
You should try high precent acril amid, like 15%

Look here
http://www.proteinchemist.com/tutorial/sdspage.html

Yoram

------------------------------------------------------------------------
This message was sent using IMP, the Webmail Program of Haifa University



More information about the Methods mailing list