Advice on TA cloning vectors
(by mlsulliv At wisc.edu)
Tue Nov 28 09:48:21 EST 2006
On Nov 27, 2006, at 8:36 PM, Jose de las Heras wrote:
> "Mauricio Realpe" <realpeqm At niaid.nih.gov> wrote in message
> news:mailman.177.1164674864.19683.methods At net.bio.net...
>> Hi. Wondering if anyone have had experience with such kind of
>> vectors and
>> could suggest to me any specific brand/product ?, stratagene ?,
>> ?, which would be more convenient for longer fragments ? (1.1 -
>> 1.5 Kbps).
>> TIA, Regards, Mauricio.
>> Mauricio Realpe. VF.
> I've been using pGEM-T Easy (promega) for all sorts of stuff...
> simple and
I've been pretty satisfied with this product as well (I have no
affiliation with Promega). I find I can scale the reaction back 2-
fold (5 ul final volume) to make the kit go farther.
Michael L. Sullivan
Plant Research Molecular Geneticist
US Dairy Forage Research Center
1925 Linden Drive West
Madison, WI 53706
(608) 890-0046 (Phone)
(608) 890-0076 (FAX)
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