how to crosslink dna binding proteins?
ben.long at yourfinger.anu.edu.au
Tue Oct 3 01:48:03 EST 2006
Yuval Ebenstein wrote:
> Hi all,
> First let me apologize if i'm not using the right terminology. I'm a
> physical chemist doing his first steps in biology....
> I would like to react CAP protein to a piece of DNA with a specific
> binding site, and then cross link and purify so that I can deposit it
> on a surface and image it with an Atomic Force Microscope (AFM).
> Can someone point out a simple but efficient crosslinking and
> purification method?
Formaldehyde is a handy and easy to use, reversible cross-linker which
you can use for this purpose. Do a simple lit search on the topic. I
don't have any specific refs at the moment. However, I know it is often
used to cross-link proteins to DNA.
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