how to crosslink dna binding proteins?

Bean Long ben.long at yourfinger.anu.edu.au
Tue Oct 3 01:48:03 EST 2006


Yuval Ebenstein wrote:
> Hi all,
> First let me apologize if i'm not using the right terminology. I'm a 
> physical chemist doing his first steps in biology....
> I would like to react CAP protein to a piece of DNA with a specific 
> binding site, and then cross link and purify so that  I can deposit it 
> on a surface and image it with an Atomic Force Microscope (AFM).
> Can someone point out a simple but efficient crosslinking and 
> purification method?
> Thanks.
> Yuval
> 

Hi Yuval,

Formaldehyde is a handy and easy to use, reversible cross-linker which 
you can use for this purpose.  Do a simple lit search on the topic.  I 
don't have any specific refs at the moment.  However, I know it is often 
used to cross-link proteins to DNA.

Cheers,
-- 
Bean

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