SDS-PAGE - Dimer formation after boiling?
ben.long at yourfinger.anu.edu.au
Tue Oct 3 19:39:46 EST 2006
I seem to have a protein forming a dimer on SDS-PAGE after boiling. I
usually pre-treat my samples to 70 °C for a few minutes prior to loading
and seem to end up with both monomers and dimers, but on boiling I seem
to get dimer formation only. Is this common? Any suggestions on what's
going on and how I can get monomers only?? I use DTT to reduce but have
seen the same results with mercapto. Haven't tried omitting a reducing
Any comments welcome.
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