How to measure Cy5-primer concentration ??

Peter Ellis pjie2 at cam.ac.uk
Thu Oct 5 12:23:32 EST 2006


DK wrote:
> In article <i6SgkQB0SMJFFARd at abuse.plus.com>, Duncan Clark
> <news at genesysltd.co.uk> wrote:
>> Historians believe that in newspost
>> <1159968940.031845.245910 at i42g2000cwa.googlegroups.com> on Wed, 4 Oct
>> 2006, chovek69 <ivanoov at gmail.com> penned the following literary
>> masterpiece:
>>> or it does
>>> not absorb at 260nm.
>>
>> Cy5 absorbs at 646. Ignore the fact that the oligo has Cy5 on there
>> and simply measure 260.
>
> Cy5 almost certainly absorbs srongly at 260-280 (two indole rings).

It doesn't.  I regularly quantitate Cy-labelled microarray probes, and the 
Cy5 signal does not interfere with the DNA signal.

Peter 




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