protocol for native gel!!!

[arikbar from pob.huji.ac.il]

Hi members !!!
I need a good protocol for native gel that will be followed by western blot. I'm
trying to find a receptor which is composed of five different subunits and also
has a transmembrane domain (for each subunit). I don't know how to calculate
the pI of the full receptor so any suggestions may help.
Thanks , Arik


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