PCR Design

Jose de las Heras josenet at tiscali.co.uk
Mon Oct 9 13:59:54 EST 2006


"Christian Praetorius" <prae at gmx.net> wrote in message 
news:4out8tFgct7gU2 at individual.net...
> "Jose de las Heras" <josenet at tiscali.co.uk> wrote:
>
>>I normally take into account the whole oligo (typically 14-16 or so bp 
>>plus
>>the recognition site I want to add)
>
> And keep in mind, that some recognition sites need additional flanking
> bases to work properly.
>
> Christian

True!

although an intermediate cloning in something like pGEM-T makes things 
easy... assuming the polymerase you're using adds Ts at high frequency.

Jose 




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