CLONING PROBLEMS-HELP

ekta jindal via methods%40net.bio.net (by ekta_saggi24 from yahoo.com)
Sat Oct 14 05:19:18 EST 2006


Dear Sir/Mam
   
  I am making a construct with pEGFP vector.I am ligating 2.7Kb isert with the vector(4.7Kb). I am getting the positive result by insert-specific primers. But i am able to isolate DNA to release the fragment to confirm the clone. The recombinant plasmid is growing very less in LB media. What could be the problem.
  Could u please suggest me something.
   
  Thanks 

 		
---------------------------------
Stay in the know. Pulse on the new Yahoo.com.  Check it out. 


More information about the Methods mailing list