CLONING PROBLEMS-HELP

[ekta jindal]

Dear Sir/Mam
   
  I am making a construct with pEGFP vector.I am ligating 2.7Kb isert with the vector(4.7Kb). I am getting the positive result by insert-specific primers. But i am able to isolate DNA to release the fragment to confirm the clone. The recombinant plasmid is growing very less in LB media. What could be the problem.
  Could u please suggest me something.
   
  Thanks 

 		
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