Concentrating adenovirus by ultracentrifugation

Zhonglin Chai via methods%40net.bio.net (by Zhonglin.Chai from baker.edu.au)
Wed Oct 18 21:23:16 EST 2006


Normally we purify (and concentrate, of course) adenovirus by double CsCl banding. 

Zhonglin Chai


-----Original Message-----
From: methods-bounces from oat.bio.indiana.edu
[mailto:methods-bounces from oat.bio.indiana.edu]On Behalf Of Tom Anderson
Sent: Thursday, 19 October 2006 8:57 AM
To: Ye Olde Methods and Reagents List
Subject: Concentrating adenovirus by ultracentrifugation


Evening all,

Has anyone here concentrated adenoviruses, or any other kind of virus or
similar, by ultracentrifugation? If so, what product did you use, and in
particular, what pore size of membrane?

I'm doing this reasonably successfully using 100 kDa MWCO Amicon Ultras;
however, i get a lot of junk in my retentate, which i assume is serum
proteins, so i'd like to move to a larger pore size, to let that through.
However, i have no idea what molecular mass-based pore sizes mean in
nanometre terms; the various manufacturers' websites are not a lot of use
(notable exception: Pall [1]).

Thanks,
tom

[1] http://www.pall.com/laboratory_7046.asp

-- 
Tom Anderson, MRC Laboratory for Molecular Cell Biology, UCL, London WC1E 6BT
(t) +44 (20) 76797264   (f) +44 (20) 76797805   (e) thomas.anderson from ucl.ac.uk

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