low molarity borate and acetate agarose gels

Ho-Leung Ng via methods%40net.bio.net (by holeung from berkeley.edu)
Thu Oct 19 16:03:05 EST 2006


     What are people's experiences using the Brody and Kern (Biotechniques
36:214 and 37:598) low molarity sodium borate and lithium acetate
agarose gels? They do run very fast with little heat, but we often
get very smeared bands, with borate performing better than acetate.
There is a lot of band quality variability from run to run. Any tips
would be appreciated!


ho
UC Berkeley



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