Stripping Solution

Pow Joshi via methods%40net.bio.net (by pow.joshi from gmail.com)
Mon Oct 23 16:20:20 EST 2006


On 10/15/06, Christian Praetorius <prae from gmx.net> wrote:
> Maryam <mzargh from yahoo.ca> wrote:
>
> >Does anybody know if NaOH solution can be used as a stripping solution? if
> So, what should be the final concentration?

you might want to add some beta mercaptoethanol or DTT as well ...
this would cleave the -S-S- linkages in the antibodies (again assuming
you want to strip western blots)

pow
>
> What do you want to strip?
>
> Christian
>
> --
> X-no-Sig: yes
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