Extraction of protein using trizol

IanMc via methods%40net.bio.net (by zebedeeboy from hotmail.com)
Fri Oct 27 04:00:05 EST 2006


Hi,

We are trying to fractionate gut samples into RNA/DNA/protein using the 
protocols in the Trizol handbook. The procedure works OK but we are having 
difficulty resuspending the protein pellet at the end of the process 1% SDS 
or 1M Tris pH10.4 has not done the trick. We are about to try the urea/CHAPS 
protocol. Does anyone have any other ideas on how to bring the pellet back 
into solution?

TIA

Ian Mc 




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