gel filtration chromatography

Ruben Martinez Buey rmartinez at cib.csic.es
Fri Sep 8 08:56:03 EST 2006


Dear All,
I am tryingto crystalize a protein that oligomerizes heterogenously (it
forms mostly dimers but also trimers, tetramers, etc...) as it can be
easily seen by gel filtration. The thing is that when I add Calcium to
the solution, I can see a mayoritary peak that would correspond to
dimers but this peak is wider than before. What could be the cause of
this peak widenning?
Thank you very much in advance.
Bests,
Ruben

IlohaCIB



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