gel filtration chromatography

Ruben Martinez Buey rmartinez at
Fri Sep 8 08:56:03 EST 2006

Dear All,
I am tryingto crystalize a protein that oligomerizes heterogenously (it
forms mostly dimers but also trimers, tetramers, etc...) as it can be
easily seen by gel filtration. The thing is that when I add Calcium to
the solution, I can see a mayoritary peak that would correspond to
dimers but this peak is wider than before. What could be the cause of
this peak widenning?
Thank you very much in advance.


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