RT PCR of long template

Pow Joshi pow.joshi at gmail.com
Fri Sep 29 11:46:12 EST 2006


Hi,

I am trying to clone a 3.2 kb cDNA with RT PCR .... while reading on
the topic, I realised that the PCR might get difficult due to the
sheer length (assuming I have very high quality RNA :)....
I would appreciate any input on strategies used for this type of cloning.

thanks much

pow



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