co-existence of expression vector and promoterless reporter vector in the same cells

[Trond Erik Vee Aune]

waikhay wrote:

> Hi all,
> 
> I am currently working on cloning and expressing a bacterial regulatory
> protein using an expression vector to test for its binding to a bacterial
> promoter which is cloned into a promoterless reporter vector that can
> ideally co-exist with the expression vector in the same E. coli cells.
> However, the expression vectors (e.g. pQE seris by Qiagen and pET series by
> Novagen) and the promoterless reporter vectors (e.g. pZsGreen1-1 and
> pDsRed-Express-1 by Clontech) that I found share the origin of replication
> which falls into the same plasmid incompatibility group. Does anyone out
> there have an idea of which expression and promoterless reporter vectors I
> can use that can also co-exist in the same cell? Any form of help would be
> greatly appreciated!!

For high expression in E.coli and other gram negative bacteria I can 
recommend our own in-house broad-host expression vectors. They are based 
on the RK2-replicon (IncP) and should therefore be compatible with most 
commercial vector systems.

For more info:
http://tinyurl.com/383ofv
http://tinyurl.com/2vyrnw

Best regards,
Trond Erik

-- 
Trond Erik Vee Aune
Department of Biotechnology, NTNU
http://www.biotech.ntnu.no/molgen

- Must be sad being a dyslectic, agnostic insomniac, lying
   awake during the night, wondering if there really is a dog