exotoxin A

[Ruhaifa Mahmoud]

Dear all,
   
  I am a postgraduate student in University of Jordan. I am making research on conjugate vaccine for Pseudomonas aeruginosa using exotoxin A as a carrier protein. Unfortunately, I was not lucky enough to isolate the protein after many trials. 
  I have worked on Pseudomonas aeruginosa IATS 11, while the main reference by Liu, used PA 103 (which i think the same). In addition, i have some problems in isolation due to effect of iron -in D.W. and media- on exotoxin A expression. 
  Any idea could be of great help.
   
  Thanks in advance.

       
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