Fluorescent labeling of Oleic Acid and/or Triglycerides

[Sharon Waldrop]

Hello,

I am looking for a procedure/protocol for labeling Oleic Acid and/or Triglycerides. I do not want to use a kit (too expensive). I am looking at the exocytosis utilizing TIRF microscopy and Nile Red is too bright and stains the lipid bylayer. I can look at red and green florescence with the TIRF microscope. Thank you for your help.



Shar Waldrop
Senior Research Associate
University of Texas Southwestern Medical Ctr
Department of Internal Medicine
Division of GastroIntestinal - MC1951
5323 Harry Hines Blvd.
Dallas, Texas  75390-1951
Dr. Gordan Kilic's Lab; J05.114
sharon.waldrop from UTSouthwestern.edu
LAB: 214.648.2063
CELL: 214.450.8086