Glutathion OD readout fluctuates
Josmar
via methods%40net.bio.net
(by josmarlangner from yahoo.de)
Thu Apr 19 05:00:34 EST 2007
no, it is not the cuvettes, water behaves normally and trying the
nanodrop could be worthwile but I don' think it will bring me further.
One photometer I used refused to blank at all and trying the second
one revealed the reason, i.e. the heavy fluctuations.
Josmar
On 19 Apr., 11:33, Tim <gerardine.pers... from gmail.com> wrote:
> Of course you've ruled out that there is a variability in the cuvettes?
> Have you tried to just measure water in a couple of replicates, in those
> cuvettes you use?
> Do you have access to a NanoDrop spectrometer? They work without
> cuvettes, and consume a lot less sample (1-2 µl) too.
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