Glutathion OD readout fluctuates

Michael Sullivan via methods%40net.bio.net (by mlsulliv from wisc.edu)
Thu Apr 19 16:07:42 EST 2007


> If you measure the UV absorption spectrum of your buffer (using a  
> water blank, which should have no absorption), you'll see why you  
> can't use your buffer as a blank. On my spec, the A at 280 nM of  
> 0.2% triton X-100 is nearly 3 abs. units and a 10 mM solution of  
> glutathione is beyond the limit of the instrument (~3 Abs. units).

I meant the absorbance of glutathione at 220 nm is off scale.
---
Michael L. Sullivan
Plant Research Molecular Geneticist
US Dairy Forage Research Center
ARS-USDA
1925 Linden Drive West
Madison, WI 53706
(608) 890-0046 (Phone)
(608) 890-0076 (FAX)



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