Glutathion OD readout fluctuates

Michael Sullivan via (by mlsulliv from
Thu Apr 19 16:07:42 EST 2007

> If you measure the UV absorption spectrum of your buffer (using a  
> water blank, which should have no absorption), you'll see why you  
> can't use your buffer as a blank. On my spec, the A at 280 nM of  
> 0.2% triton X-100 is nearly 3 abs. units and a 10 mM solution of  
> glutathione is beyond the limit of the instrument (~3 Abs. units).

I meant the absorbance of glutathione at 220 nm is off scale.
Michael L. Sullivan
Plant Research Molecular Geneticist
US Dairy Forage Research Center
1925 Linden Drive West
Madison, WI 53706
(608) 890-0046 (Phone)
(608) 890-0076 (FAX)

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