Methods Digest, Vol 23, Issue 21

[Virash Gupta]

Make primer solution:
dissolve whole of 150nM into 1500 microlitre of water or 1mM Tris to get 100
micromole solution. Is not it simple.Tell others.
 Dr V K Gupta
Insect Mol Biology Lab
Punjab Agricultural University
Ludhiana-India 141004


On 4/25/07, methods-request from oat.bio.indiana.edu <
methods-request from oat.bio.indiana.edu> wrote:
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> Today's Topics:
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>   1. make primer solution? (TRAN HO QUANG)
>   2. Re: make primer solution? (Tim)
>   3. Re: make primer solution? (Duncan Clark)
>   4. 1D gel analysis (Siddhartha Parida)
>
>
> ----------------------------------------------------------------------
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> Message: 1
> Date: Tue, 24 Apr 2007 19:14:26 -0700 (PDT)
> From: TRAN HO QUANG <quangtranho from yahoo.com>
> Subject: make primer solution?
> To: methods from magpie.bio.indiana.edu
> Message-ID: <870240.46383.qm from web43139.mail.sp1.yahoo.com>
> Content-Type: text/plain; charset=ascii
>
> Can netters tell me how to make primer solution from lyophilized (from
> supplier company) to 100 uM (stock solution). Let say primer has 150 nmoles
> as recorded from company.
>
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> ------------------------------
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> Message: 2
> Date: Wed, 25 Apr 2007 09:31:13 +0200
> From: Tim <gerardine.persson from gmail.com>
> Subject: Re: make primer solution?
> To: methods from net.bio.net
> Message-ID: <462f03c2$1 from griseus.its.uu.se>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Let's see... What are the equations in every textbook?
>
> concentration = moles / liter
>
> In your case:
> concentration (you want) = 100 umol / liter
> moles (you have) = 150 nmol
>
> turn the equation around to say
>
> liter = moles / concentration
>
> Now get out your calculator and divide
>
> 150 nmol / (100 umol / liter)
>
> Make sure you don't drop the unit prefixes nano and micro (n and u).
>
> TRAN HO QUANG wrote:
> > Can netters tell me how to make primer solution from lyophilized (from
> supplier company) to 100 uM (stock solution). Let say primer has 150 nmoles
> as recorded from company.
>
>
> ------------------------------
>
> Message: 3
> Date: Wed, 25 Apr 2007 09:30:10 +0100
> From: Duncan Clark <blackhole from abuse.plus.com>
> Subject: Re: make primer solution?
> To: methods from net.bio.net
> Message-ID: <fKoQTalSGxLGFAYm from abuse.plus.com>
> Content-Type: text/plain;charset=us-ascii;format=flowed
>
> Historians believe that in newspost
> <mailman.853.1177477727.5139.methods from net.bio.net> on Tue, 24 Apr 2007,
> TRAN HO QUANG <quangtranho from yahoo.com> penned the following literary
> masterpiece:
> >Can netters tell me how to make primer solution from lyophilized (from
> supplier company) to 100 uM (stock solution). Let say primer has 150 nmoles
> >as recorded from company.
>
> Strangely I find this simple calculation confuses an awful  lot of
> people.
>
> I always think like the following:
>
> 150nMoles is 150,000pMoles
>
> 100uM stock is 100pM per ul
>
> Now use calculator.
>
> Duncan
> --
> I love deadlines. I especially like the whooshing noise they make as
> they go flying by.
>
> Duncan Clark
> GeneSys Ltd.
>
>
> ------------------------------
>
> Message: 4
> Date: Wed, 25 Apr 2007 18:58:10 +0530
> From: "Siddhartha Parida" <sidharth4 from gmail.com>
> Subject: 1D gel analysis
> To: methods from magpie.bio.indiana.edu
> Message-ID:
>        <f518ad060704250628v34b76cc0od100ffb6c402f1a0 from mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1; format=flowed
>
> Can anyone help me finding a free 1D gel analysis sofware in the internet
> --
> Sidharth Parida
> Senior Faculty
> Center for biotechnology and Research
> Neelachal Inst of Medical Sciences
> O.C.H.C Complex, Block - III (Near Ram Mandir)
> Janpath,Unit-3,Bhubaneswar- 751001
> ORISSA
> 09437089337(M)
>
> sidharth4 from gmail.com
> www.nimsindia.com
>
>
>
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> End of Methods Digest, Vol 23, Issue 21
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