HPLC DNA purification

peter via methods%40net.bio.net (by peter.ianakiev from gmail.com)
Mon Apr 30 10:13:52 EST 2007


On Apr 30, 10:51 am, Duncan Clark <blackh... from abuse.plus.com> wrote:
> Historians believe that in newspost
> <1177940383.017397.79... from p77g2000hsh.googlegroups.com> on Mon, 30 Apr
> 2007, peter <peter.ianak... from gmail.com> penned the following literary
> masterpiece:
>
> > I wonder if
> >I can ligate them directly or I have first to purify and remove the
> >organic solvents (or use the vacuum ).
>
> Vacuum dry which will remove both the TEAA and acetonitrile. Re-suspend
> in TE and go from there.
>
> Duncan
> --
> I love deadlines. I especially like the whooshing noise they make as
> they go flying by.
>
> Duncan Clark
> GeneSys Ltd.

Thanks Duncan,
Have a question - do I have to really dry out, or just let it vacuum
for 10 min, and let the volatile solvents go first, what is your take?
Peter



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