inert dye for real-time pcr

[Tim Fitzwater]

"I'm looking for an inert dye to add to my colorless real-time pcr
mixes to be able to check the performance of a pipetting robot."

 

There are published reports of two different red dyes used for this
purpose.

 

1) 0.01% Phenol red (R. J. Klebe, S. A. Rodriguez, M. L. VerBeek and T.
A. 

Giambernardi, 1999.  Simple method for "hot-start" RT-PCR.  

BioTechn. 27(6):1108-1110.)

 

2) Carl Wittwer's laboratory uses 0.1 mM Cresol Red for the same purpose


(The Rapid Cyclist, Spring, 1994, page 9).

 

Tim