qPCR Efficiency

Yvonne Couch via methods%40net.bio.net (by yvonne.couch from dpag.ox.ac.uk)
Thu Aug 2 12:04:14 EST 2007


Hi all,

I'm running some qPCR at the moment and trying to calculate efficiency using
my standard curve but it always ends up around -1.4, which is around 300%
efficiency which definitely isn't right!  Any ideas what has gone amiss?

Cheers

Yvonne



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