precipitating bacteriophage-E79
Kanwaldeep Dhaliwal
via methods%40net.bio.net
(by kdhaliwa from staffmail.ed.ac.uk)
Mon Aug 6 14:17:20 EST 2007
Hi all
can anyone help? Iam having trouble precipitating E79 phage (specific
for PAO1).
i have lystaes with a titer of 10^22 per ml of E79 to which i added
some chloroform and kept at 4 degrees C, and am trying to PEG
precipitate with 20% PEG 800 and 2.4 M NACL in PBS
then on ice for 30 mins and spin at 9000G for 20 mins
i never get a pellet I can see
Am i doing something very stupid
thanks
Kev
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