precipitating bacteriophage-E79

Kanwaldeep Dhaliwal via methods%40net.bio.net (by kdhaliwa from staffmail.ed.ac.uk)
Mon Aug 6 14:17:20 EST 2007


Hi all

can anyone help? Iam having trouble precipitating E79 phage (specific  
for PAO1).

i have lystaes with a titer of 10^22 per ml of E79 to which i added  
some chloroform and kept at 4 degrees C, and am trying to PEG  
precipitate with 20% PEG 800 and 2.4 M NACL in PBS

then on ice for 30 mins and spin at 9000G for 20 mins

i never get a pellet I can see

Am i doing something very stupid

thanks

Kev



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