How to identify lanes on large agarose gels?

[chovek69]

On Aug 7, 1:11 am, Peter Frank <peter_fran... from yahoo.de> wrote:
> Michael Sullivan <mlsul... from wisc.edu> wrote:
> >Why not include something bigger in size than you expect for your PCR  
> >products in every lane, e.g. linerarized plasmid.
>
> Yes, that would probably work. Do you think that would impair the
> samples somehow? If yes, how about adding that plasmid  into each well
> shortly before I stop the gel run so that the linearized plasmid would
> only run by itself and only a small distance into the gel?
>
> Peter

I think with a little tweaking of settings on the camera/photodocum.
system (especially brightness and contrast, aperture and shutter) you
should be able to see the wells on the gel. If you only interested in
the yes/no result there is no need for rulers and ladders, only
positive control of expected size.