How to efficiently transfect U937 cells with a plasmid vector expressing shRNA

[Prof. Piero Sestili]

We are trying to transfect U937 cells with a plasmid vector expressing
shRNA. However we are obtaining a very low transfection efficiency using
either electroporation (which is not the best solution since we need
that transfected cells retain their viability for some weeks) or
lipofectin.  Is there anybody who knows some nice alternative method or
trick to increase the efficiency?  thanks a lot,

 

Piero 

 

Prof. Piero Sestili
Istituto di Farmacologia e Farmacognosia e
Centro di Ricerca sull'Attività Motoria
Università degli Studi di Urbino "Carlo Bo"
Via "I Maggetti" 26
61029 URBINO (PU)
Tel. 0722 303414; 0722 305524 
Fax 0722 303401