qPCR NEWSLETTER - August 2007

Editor www.Gene-Quantification.info via methods%40net.bio.net (by editor from gene-quantification.info)
Thu Aug 9 09:40:06 EST 2007

qPCR NEWSLETTER - August 2007

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Dear researcher,
dear Gene Quantification page reader,

Our newsletter informs about the latest news in quantitative real-time
PCR (qPCR and qRT-PCR), which are compiled and summarised on the Gene
Quantification homepage. The focus of this newsletter issue is:

- microRNA - new LINKs
- microRNA - new microRNA application papers on microRNA page 4 and 5
- qPCR application workshop in autumn in Germany - new dates !
- qPCR Symposium USA in October/November 2007 - register now !



microRNA Definition
non-coding RNAs / DataBase
micro RNA talks and microRNA posters
Nature Genetics 38, S1 (2006) - The microRevolution
miRNA Research Education Research Center
Human MicroRNA targets
miRU - Plant microRNA Potential Target Finder
Cancer genomics:   Small RNAs with big impacts
miRU: an automated plant miRNA target prediction server
miRBase - the home of microRNA data
miRBase: microRNA sequences, targets and gene nomenclature
The microRNA Registry
A uniform system for microRNA annotation
TRC - the RNAi Consortium shRNA Library
Algorithms & Thermodynamics for Nucleic Acid Folding, Hybridization &
Melting Profile Prediction
TUTORIAL:  Check out an illustrated tutorial on miRNA (by Ambion)


microRNA  (miRNA)   &   quantitative real-time RT-PCR (NEW page 4):


Strategies to determine the biological function of microRNAs.
Genomics of microRNA.
Tracing microRNA patterns in mice.
Target mimics modulate miRNAs.
Target mimicry provides a new mechanism for regulation of microRNA
Lessons from Nature: microRNA-based shRNA libraries.
Identification of Differentially Expressed MicroRNAs by Microarray: A
Possible Role for MicroRNA Genes in Pituitary Adenomas.
RNA polymerase III transcribes human microRNAs.
Identification by Real-time PCR of 13 mature microRNAs differentially
expressed in colorectal cancer and non-tumoral tissues.
Rational Probe Optimization and Enhanced Detection Strategy for
MicroRNAs Using Microarrays.
Potential mRNA Degradation Targets of hsa-miR-200c, Identified Using
Informatics and qRT-PCR.
Real-time expression profiling of microRNA precursors in human cancer
cell lines.
Multiplexing RT-PCR for the detection of multiple miRNA species in
small samples.
An oligonucleotide microchip for genome-wide microRNA profiling in
human and mouse tissues.
Microarray analysis shows that some microRNAs downregulate large
numbers of target mRNAs.
MicroRNA expression profiling using LNA-modified probes in a liquid-
phase bead-based array.
miRCURY™ LNA research tools for microRNA.


microRNA  (miRNA)   &   quantitative real-time RT-PCR (NEW page 5):


Editorial - The microRevolution.
Glimpses of a Tiny RNA World.
Functions of microRNAs and related small RNAs in plants.
Evidence that microRNAs are associated with translating messenger RNAs
in human cells.
How microRNAs control cell division, differentiation and death.
microRNA target predictions in animals.
Human let-7a miRNA blocks protein production on actively translating
Computational identification of microRNA targets.
Incorporating structure to predict microRNA targets.
220-plex microRNA expression profile of a single cell.
Intronic microRNA precursors that bypass Drosha processing.
MicroInspector: a web tool for detection of miRNA binding sites in an
RNA sequence.
Target labelling for the detection and profiling of microRNAs
expressed in CNS tissue using microarrays.
A brain-specific microRNA regulates dendritic spine development.


Update of the Webinar page

A lot of interesting TALKs, WEBINARs, SLIDE SHOWs, and PODCASTs from
various speakers, biotec companies, qPCR Events, and international
journals (Nature and Science) are FREE for download. Have a look and
you will definitely something interesting for your scientific work !




Protocols online is a resource for protocols, including authoritative,
peer-reviewed 'protocols and an interactive network.


Upcoming Events World-wide academic and commercial qPCR Events

Symposia, Meetings, Conferences, Workshops, Seminars, Online-Seminars,
qPCR Education Program, ...etc..
Please submit your qPCR event here  =>  events from gene-


qPCR Symposium USA

Symposium Focus:
Markers, Stem Cells, Single Cell, siRNA, miRNA, Diagnostics, Immuno-
qPCR, Expression Profiling,
Poster Presentation, Workshops in qPCR


TATAA Biocenter Germany - qPCR Application workshops

At the TATAA Biocenter Germany we offer qPCR application workshops,
the 3-day Core Module and a 2-day Biostatistics Module.  qPCR courses
are held in regularly in Göteborg, Sweden, in English and in Freising-
Weihenstephan, Germany, in German and English, and in Prague, Czech
Republic in English and Czech. Depending on the occasion the workshop
language and the different prices may apply. Further customized
workshops and specialized trainings will be held as well across Europe
and world-wide. TATAA Biocenter Germany courses are held in
cooperation with the Institute of Physiology, located at the Technical
University of Munich, in Freising-Weihenstephan, near Munich, very
close to the Munich Airport (MUC). For more information and to
register for the qPCR application workshops, please see our web page:

Course Occasions 2007:

3-day qPCR Core Module  (Mon. - Wed.)    and     2-day BioStatistics
Module (Thu. - Fri.)

22 - 26th October 2007  (in Freising, Germany, English language)
26 - 30th November 2007 (in Freising, Germany, Kurs wird in DEUTSCH
gehalten, German language)

Please register here  =>  http://www.tataa.com/Courses/Courses.html


Forward Please send the qPCR NEWS to further scientists and friends
who are interested in qPCR !

Best regards,

Michael W. Pfaffl
responsible Editor of the Gene Quantification Pages


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