Electroporating tissue in suspension was Re: R to DK: How to
efficiently transfect U937 cells with a plasmid vectorexpressing shRNA
Tom Anderson
via methods%40net.bio.net
(by ucgatan from ucl.ac.uk)
Fri Aug 10 08:43:38 EST 2007
On Thu, 9 Aug 2007 nanooe from gmail.com wrote:
> Did you try Microporation? www.microporator.com. Slightly different take
> on electroporation so may have advantages for explants?
Funny you should mention that! We got an advert from them, and apparently
there's someone in a nearby department with one. I sent them an email a
few weeks ago, but never heard back. One of the things on my to-do list
for today is to chase that up.
tom
> On Aug 8, 4:00 pm, Tom Anderson <ucga... from ucl.ac.uk> wrote:
>
> > Has anyone ever tried, and moreover had any success at, electroporating
> > chunks of tissue in suspension?
--
Tom Anderson, MRC Laboratory for Molecular Cell Biology, UCL, London WC1E 6BT
(t) +44 (20) 76797264 (f) +44 (20) 76797805 (e) thomas.anderson from ucl.ac.uk
More information about the Methods
mailing list