Electroporating tissue in suspension was Re: R to DK: How to efficiently transfect U937 cells with a plasmid vectorexpressing shRNA

Tom Anderson via methods%40net.bio.net (by ucgatan from ucl.ac.uk)
Fri Aug 10 08:43:38 EST 2007


On Thu, 9 Aug 2007 nanooe from gmail.com wrote:

> Did you try Microporation? www.microporator.com. Slightly different take
> on electroporation so may have advantages for explants?

Funny you should mention that! We got an advert from them, and apparently
there's someone in a nearby department with one. I sent them an email a
few weeks ago, but never heard back. One of the things on my to-do list
for today is to chase that up.

tom

> On Aug 8, 4:00 pm, Tom Anderson <ucga... from ucl.ac.uk> wrote:
>
> > Has anyone ever tried, and moreover had any success at, electroporating
> > chunks of tissue in suspension?

-- 
Tom Anderson, MRC Laboratory for Molecular Cell Biology, UCL, London WC1E 6BT
(t) +44 (20) 76797264   (f) +44 (20) 76797805   (e) thomas.anderson from ucl.ac.uk



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