Electroporating tissue in suspension was Re: R to DK: How to efficiently transfect U937 cells with a plasmid vectorexpressing shRNA

[Joe Doe]

In article <mailman.566.1186588023.11350.methods from net.bio.net>,
 Tom Anderson <ucgatan from ucl.ac.uk> wrote:

> While we're on the subject of electroporation,
> 
> Has anyone ever tried, and moreover had any success at, electroporating
> chunks of tissue in suspension? I'm working with an explant culture
> system, and it would be incredibly useful to be able to put genes into the
> explants before culturing them. At present, i can do this with viruses,
> but this is a bit cumbersome, and i haven't got any chemical transfection
> method, or nucleofection, to work.
> 
> Dissociating the explants and electroporating the cells is a non-starter -
> the cells don't come out right unless the explants are intact.
> 
> tom

How about using a gene gun:

check out BioRad Helios gene gun.

Roland