Electroporating tissue in suspension was Re: R to DK: How to efficiently transfect U937 cells with a plasmid vectorexpressing shRNA

Tom Anderson via methods%40net.bio.net (by ucgatan from ucl.ac.uk)
Sun Aug 12 10:26:25 EST 2007


On Fri, 10 Aug 2007, Joe Doe wrote:

> In article <mailman.566.1186588023.11350.methods from net.bio.net>,
>  Tom Anderson <ucgatan from ucl.ac.uk> wrote:
>
> > Has anyone ever tried, and moreover had any success at, electroporating
> > chunks of tissue in suspension?
>
> How about using a gene gun:
>
> check out BioRad Helios gene gun.

Another thing i haven't tried. Will look into it - i think we might even
have an old one somewhere in the building.

tom

-- 
Tom Anderson, MRC Laboratory for Molecular Cell Biology, UCL, London WC1E 6BT
(t) +44 (20) 76797264   (f) +44 (20) 76797805   (e) thomas.anderson from ucl.ac.uk


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