Electroporating tissue in suspension was Re: R to DK: How to efficiently transfect U937 cells with a plasmid vectorexpressing shRNA

[nanooe from gmail.com]

On Aug 10, 2:43 pm, Tom Anderson <ucga... from ucl.ac.uk> wrote:
> On Thu, 9 Aug 2007 nan... from gmail.com wrote:
>
> > Did you try Microporation?www.microporator.com. Slightly different take
> > onelectroporationso may have advantages for explants?
>
> Funny you should mention that! We got an advert from them, and apparently
> there's someone in a nearby department with one. I sent them an email a
> few weeks ago, but never heard back. One of the things on my to-do list
> for today is to chase that up.
>
> tom
>
> > On Aug 8, 4:00 pm, Tom Anderson <ucga... from ucl.ac.uk> wrote:
>
> > > Has anyone ever tried, and moreover had any success at, electroporating
> > > chunks of tissue in suspension?
>
> --
> Tom Anderson, MRC Laboratory for Molecular Cell Biology, UCL, London WC1E 6BT
> (t) +44 (20) 76797264   (f) +44 (20) 76797805   (e) thomas.ander... from ucl.ac.uk

Would be interested in finding out how you get along. Looks interesting