long PCR

dana hattab via methods%40net.bio.net (by z_hattab from yahoo.com)
Tue Aug 28 12:17:41 EST 2007


Hi,
   
  did any body perform long PCR the products between 10-12 Kb, what was the main problems you face? dose the concentration of the genomic DNA affects the results?  any advises or recomended reading about the procedure.
   
  thanx

       
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