nuclear extracts and 2D

Jorge Pinto via methods%40net.bio.net (by ijpinto from ibmc.up.pt)
Thu Aug 30 11:58:35 EST 2007


Hello,
 
Can anybody give me a reliable protocol for isolation of nuclear extracts
from HepG2 cells and their use in 2D electrophoresis? Thanks in advance.
 
Jorge
 
Jorge P Pinto, PhD
Iron Genes and Immune System
Cell Culture and Genotyping (Coordinator)
Institute for Molecular and Cell Biology
Rua do Campo Alegre, 823
4150-180 Porto
Portugal
Phone: +351-226074956
 


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